|
Name |
Exosomal RNA and Protein Extraction Kit |
|
Cat. # |
P200 |
|
Application |
For extracting both exosomal RNA and/or exosomal protein from pure exosome isolated by our Exosome Isolation Kits (Cat.#: P100, P101, P120, P121). This product is for research use only. |
|
Product Size |
20 reactions |
|
Yield |
In each reaction, from 50 µL ~ 100 µL pure exosome, 50 ~ 100 ng exosomal RNA or 150 ~ 200 µg exosomal protein can be extracted. |
|
Shipping / Storage |
Ship at 4℃, and store at -20℃ and room temperature (see component table below). Upon receiving keep all bottles upright, at proper temperature (component table below) in dark place. |
|
Shelf Life |
|
|
Manual (protocol) |
 |
|
Component |
Component |
Amount |
Storage |
|
Exosomal Protein Lysis buffer * |
2 mL |
-20℃ |
|
N1 |
5 mL |
Room temperature |
|
N2 |
1 mL |
Room temperature |
|
N3 |
2.5 mL |
Room temperature |
|
N4 |
10 mL |
Room temperature |
|
RNA Elution buffer |
0.5 mL |
Room temperature |
|
|
|
* Store at 4℃ for 7 days or aliquot and store at -20℃ for up to 3 months. |
|
Remarks |
Important: RNA is sensitive to RNase. Before starting RNA extraction, prepare clean lab bench and wipe working surface and pipettors with RNase decontamination solution, such as Ambion® RNaseZap®. Always wear clean laboratory gloves during |
